Chapterl 1: System Description LB 9507
1-2
4. Uptake/FTI measurements
In this measurement mode, a standard is used to convert patient
samples into a %-uptake and then into an FTI-value (Free Thyroid
Index).
5. Dual-Luciferase
TM
Reporter Gene Assay
This measurement is used for automatic measurement of
Promega's Dual-Luciferase
TM
Reporter Gene Assay (injection-
measurement-injection-measurement). In this measurement
mode, the quantity of light measured in RLU units over a user-
defined measuring time is produced for both measurements of
each sample. The mean value and the coefficient of variation are
generated for replicate groups. The measured data of both
measurements will be evaluated to get the normalized response.
6. Kinetic and Repeated Measurements
These measurement modes are used to follow light emission from
the sample in time. A kinetic protocol can be used to take up to
2000 data points from one measurement. During a repeated
measurement the sample will be measured several times. Each
measurement results in one measurement point.
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