LB 9507 Appendix
13
A.6 Glossary
1. Activator Reagents: The reagents required to initiate the light emission, e.g.
microperoxidase and H
2
O
2.
The starter reagents can be injected
successively from two bottles, or, pre-mixed, from one bottle.
2. Master Curve: The kit manufacturer calculates an average standard curve using
many assays of the same type (for example, measured over a
period of several days, by different operators). The parameters of
this curve are included in the kit description and entered into the
instrument by the user. This ensures that the user must not
measure a complete standard curve prior to each series of patient
samples. Prior to each measurement, however, the Master Curve
is re-adjusted by means of two calibrators.
3. FLG: Flags, appears in the printout to mark out-of-range samples.
4. ILMA: Immuno-Luminometric-Assay, corresponding to the Immuno-
Radiometric-Assay (IRMA). Basically a sandwich assay. The
measuring signal increases with rising analyte concentration.
5. Calibrators: see 2. Master Curve
6. Full Standard Series: These protocols operate without Master Curve; the standard
curve is measured individually, but may also be stored and edited
for re-use.
7. Controls: Control samples with known concentrations and thresholds.
8. Cubic Spline Function: Parts of 3rd degree polynomials continuous at the osculation
points, and also continuous in the first and second derivation.
9. LIA: Luminescence-Immuno-Assay, competitive assay corresponding
to RIA.
y
10. Logit Function: The definition is logit y = ln
1 - y
11.Logit-Log Transformation: To approximate the standard curve as closely as possible to a
linear curve. This type of transformation has to be used when the
standard curve at the upper RLU end is too flat.
Log always means ln (logarithmus naturalis).
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