LB 9507 Chapter 3: Getting Started
3-11
3.7 Reagent Supply
Depending on the requirements for the luminescence reaction, the
instrument may operate with 0, 1 or 2 injectors. Accordingly, you
have to install one or two reagent bottles. Two empty bottles are
included with the instrument. Kit manufacturers supply their
specific activator and starter reagents in suitable bottles.
Technical data about the injector system
Injector volume: 25µL - 300µL in 5µL steps
Injection time: less than 0.6 seconds
Precision: ± 3µL for the total range;
<0.5% (300µL <0.1%)
Handling of injectors
Injector lines have to be washed before starting an assay. Fill
lines with reagents. After each experiment the injector lines have
to be cleaned and the liquid has to be removed from the system.
Filling of the injector lines
Proceed as follows:
a) Switch on the instrument.
b) Make sure that the setting of the injectors is correct (see
<INSTRUMENT PARAMETERS>, section 3.8.1).
c) From the <OPERATOR FUNCTIONS> menu, select the
function <REAGENT> <PRIME> to fill up the supply lines
and pump(s).
Pumping back the reagents
After measurement, the reagents left in the injector system, can
be pumped back into the reagent bottles (see section 4.2.3,
<MANUAL UNLOAD> function in the <OPERATOR
FUNCTIONS> <REAGENT> menu).
Washing of injector lines after the measurement
After finishing measurement the injector system should be
cleaned. Injector lines are made of Teflon/Tefzel and are resistant
to a variety of different chemicals. For cleaning the lines you
should use a lye or an acid (e.g. 0.3 M NaOH or 0.3 M HCL).
Then rinse lines several times with H
2
0. Remove the liquid from
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