LUMAT LB 9507 User Manual (English)

Questions and Answers:

R
Ronald BentleyJul 31, 2025
Why is the background effect too high on my LUMAT LB 9507?
- D
  Diamond WellsJul 31, 2025
  If the background effect is too high in your LUMAT Laboratory Equipment, first check the measurement protocol for any incorrect background effect entries and change the protocol entry accordingly. Other potential causes include malfunctioning sealing elements, a contaminated measurement chamber, a defective photomultiplier or electronic unit, or fan failure; in these cases, it is best to call Technical Service. Additionally, ensure that sample tubes are not stored in direct sunlight to avoid phosphorescence and that the sample tubes are suitable and not contaminated. For more details on suitable sample tubes, see chapter 1.
I
ifordAug 4, 2025
What to do if my LUMAT LB 9507 shows 'MEMORY ERASED!'?
- S
  Steven MyersAug 4, 2025
  If your LUMAT Laboratory Equipment displays 'MEMORY ERASED!', this indicates that the memory content is incorrect, and all protocols and instrument settings have been deleted. You should re-enter the protocols. If the issue persists, call Technical Service.
K
Keith NunezAug 8, 2025
Why is the pump not injecting in my LUMAT Laboratory Equipment?
- A
  Adam ConradAug 8, 2025
  If the pump is not injecting in your LUMAT Laboratory Equipment, it could be due to a defective tubing system, which requires replacement. Alternatively, check all screw connections to ensure they are tight.
J
Joseph McclureAug 13, 2025
What to do if the injector volume is not constant in my LUMAT LB 9507?
- S
  Sarah ColonAug 13, 2025
  If the injector volume is not constant in your LUMAT Laboratory Equipment, a possible cause is a leak in the tubing system. Check the screws to ensure they are tight.
A
Aaron McphersonAug 17, 2025
Why is the rotor not turning, but the drive works, in my LUMAT LB 9507 Laboratory Equipment?
- D
  David AllenAug 17, 2025
  If the rotor isn't turning but the drive works in your LUMAT Laboratory Equipment, the measurement chamber may be soiled. In this case, it is best to call Technical Service.

LUMAT LB 9507 Specifications

General

Type	Luminometer
Interfaces	RS-232
Power Supply	100-240 V AC, 50/60 Hz
Display	LCD
Detector	Photomultiplier tube
Sensitivity	1 x 10^-15 mol ATP

Summary

Preface

Use and Function

Describes the instrument's purpose and operation in bio- and chemiluminescence assays.

Special Instructions and Precautions

Details essential safety guidelines and user responsibilities for safe operation.

1. System Description

1.1 Application

Covers the instrument's uses and capabilities in bio- and chemiluminescence applications.

1.2 Structure and Operation of the Software

Details the software's menu-based structure, operational modes, and measurement protocols.

1.3 The Principle of Measurement

Explains the underlying technology of light detection using a photomultiplier and photon counting.

1.4 Sample Tubes

Describes the required dimensions and material recommendations for sample tubes.

1.5 Data Management and Software update

Explains how measured data is stored and how software updates are managed.

1.6 Simple and Reliable Operation

Highlights the instrument's user-friendliness, multilingual support, and self-diagnostic features.

1.7 Instrument and software versions

Lists different Lumat LB 9507 instrument and software versions with order numbers.

2. Design of Lumat LB 9507

Front view

Identifies the main components visible on the front panel of the instrument.

Inside view

Shows a diagram of the internal components and their locations within the instrument.

Rear side

Details the various connections and controls located on the rear panel of the Lumat.

2.1 Keyboard

Describes the layout, function keys, and softkeys of the instrument's keyboard interface.

2.2 Display

Explains the characteristics and functions of the two-line LCD display.

2.3 Printer

Covers the functions of the built-in thermal printer for protocols and results.

2.4 Measurement Unit

Describes the structure and function of the measurement unit, including the rotor and chamber.

2.5 Reagent Storage Area

Details the location and connections for reagent bottles used with the instrument.

2.6 Variable injectors

Explains the function, volume definition, and precision of the variable injectors.

2.7 Reagent Supply

Discusses Lumat versions, injector volume settings, and the wash function.

2.8 Memory Management

Details the instrument's memory structure, storage security, and data retention.

3. Getting Started

3.1 Instrument Setup

Lists environmental and placement requirements for setting up the instrument.

3.2 Power Supply

Explains voltage detection, fuse requirements, and mains connection.

3.3 Checking the Paper Supply

Advises on checking paper supply and refers to section for changing paper.

3.4 Switching on Lumat

Describes the power-on sequence, initialization, and error indicators.

3.5 System Configuration Parameters

Explains how to access and set general operating parameters in the system configuration menu.

3.6 Instrument Parameters

Covers essential instrument settings like injector times and sensitivity calibration factors.

3.7 Reagent Supply

Discusses injector system technical data, handling, filling, and washing procedures.

3.8 Safety Precautions for Operation

Lists essential safety measures for operating the instrument to ensure user and equipment safety.

4. Structure and Operation of Software

4.1 Operation

Describes the menu-based software operation, function keys, and keyboard layout.

4.2 The Six Functions of the Main Menu

Overviews the main menu options: Measure, Protocol, Operator Functions, System Configuration, Instrument Parameter, Turn Once.

4.3 General Procedure

Provides a flowchart overview of essential instrument setup, protocol creation, measurement, and operator functions.

5. Raw Data Measurements

5.1 Introduction

Introduces the raw data measurement mode, its applications for ATP and reporter gene assays, and data output options.

5.2 Creating Raw Data Protocols

Details the process of creating, saving, and naming measurement protocols for routine use.

5.3 Editing/Deleting Protocols

Explains how to modify existing or delete unwanted measurement protocols from the instrument's memory.

5.4 Performing Raw Data Measurements

Describes how to run raw data measurements using stored protocols or direct parameter entry.

6. CUT-OFF Measurements

6.1 Introduction

Explains the purpose of cut-off measurements for classifying samples into qualitative categories based on RLU thresholds.

6.2 Creating CUT-OFF Protocols

Details the process of creating and saving measurement protocols for cut-off assays.

6.3 Editing/Deleting Protocols

Explains how to modify or remove existing cut-off measurement protocols.

6.4 Running Cut-off Measurements

Describes the step-by-step procedure for performing cut-off measurements using stored protocols.

7. Quantitative Measurements (LIA...)

7.1 Introduction

Introduces quantitative measurements for determining analyte concentrations using standard series or master curves.

7.2 Setting Up Quantitative Protocols

Details the process of creating and saving quantitative measurement protocols, including standard series or master curve options.

7.3 Editing/Deleting Protocols

Explains how to modify or remove existing quantitative measurement protocols.

7.4 Entering a Master Curve

Describes the procedure for inputting master curve data, which is used instead of a full standard curve.

7.5 Performing Quantitative Measurements

Covers the steps for performing quantitative measurements using either a complete standard curve or a master curve.

8. T-Uptake/FTI-Measurements

8.1 Introduction

Explains T-uptake and FTI measurements, including calculation equations and requirements for T4 data.

8.2 Creating T-uptake/FTI protocols

Details the process of creating and saving measurement protocols for T-uptake and FTI assays.

8.3 Editing/Deleting Protocols

Explains how to modify or remove existing T-uptake/FTI measurement protocols.

8.4 Performing T-uptake/FTI Measurements

Describes how to perform T-uptake/FTI measurements using stored protocols.

9. Dual-LuciferaseTM Reporter Gene Assay

9.1 Creating Protocols for Dual-LuciferaseTM Assay

Details the process of creating protocols for the Dual-Luciferase assay, considering injector configurations.

9.2 Editing/Deleting Protocols

Explains how to modify or remove existing Dual-Luciferase assay protocols.

9.3 Performing Dual-LuciferaseTM Reporter Gene Assay Measurements

Describes the step-by-step procedure for running the Dual-Luciferase reporter gene assay.

10. Kinetic and Repeated Measurements

10.1 Kinetic Option in Raw Data Protocol

Explains how to enable kinetic printouts for data and curves in raw data protocols.

10.2 Repeated Measurements

Introduces repeated measurements for long-term kinetics, defining time windows and intervals.

10.3 Ratemeter

Describes continuous kinetic measurements in ratemeter mode, allowing manual injections and flexible intervals.

11. OPERATOR FUNCTIONS

<REAGENT>

Covers functions for loading, refreshing, removing reagents, and washing injectors.

<PERF. TEST>

Describes the performance test procedure to assess instrument and reagent quality.

<MANUAL DATA >

Explains how to manually enter RLU values for data entry and curve generation.

<STORED DATA>

Covers retrieval, recalculation, editing, and printing of stored measurement data.

12. Connection to external computers

12.1 General

Provides general information on connecting the LB 9507 to external computers via RS 232.

12.2 RS 232 Data Output LB 9507 in Protocol-Specific Measuring Mode

Details the format and content of data output via the RS 232 interface during measurements.

12.3 Data Output in Raw Data Measurements

Describes the output format for measured RLU values in raw data measurements.

12.4 Data Output after Manual Input of RLU Values

Explains data output for calculations based on manually entered RLU values.

12.5 Data Output of Stored Assay Data

Refers to data output for stored assay data, referencing section 12.2.

12.6 Data Output of RLU/s in the Ratemeter Mode

Describes data output format for RLU/s values in ratemeter mode.

13. Maintenance

13.1 Supplying Paper

Details the procedure for loading a new paper roll into the instrument's thermal printer.

13.2 Replacing the Mains Fuse

Explains the steps for safely replacing the mains fuse located on the rear panel.

13.4 Cleaning the Injector System

Describes the process for cleaning the injector lines with wash solution or priming with reagents.

13.6 Changing the Air Filter

Instructions for cleaning or replacing the air filter unit monthly.

13.7 Checking the Hose Connections

Describes how to check hose connections for leaks, indicating salt deposits as signs of leaks.

14. Troubleshooting

14.1 Error Messages during Operation

Lists common display error messages, their likely causes, and recommended solutions.

14.2 Injector System Malfunction

Addresses issues like pump not injecting or inconsistent injector volumes and their potential causes/solutions.

14.3 Other Errors

Covers other potential errors such as tube jamming or display issues after power-on.

14.4 Default Values

Lists the default instrument parameters stored in the instrument's memory.

15. Technical Specifications of LB 9507

Injectors

Specifies the number of injectors the instrument can be equipped with: 0, 1, or 2.

Injector volume

Details the adjustable range and step size for injector volumes.

Sample tubes

Describes the dimensions, tolerances, and recommendations for sample tubes.

Measuring time

Specifies the configurable range for measurement time.

Software

Lists the various measuring modes and protocols supported by the Lumat software.

Hardware

Details the instrument's hardware specifications, including microprocessor and memory.

Detector

Specifies the characteristics of the photomultiplier detector, including resolution and sensitivity.

Luminescence immunoassay protocols

Outlines parameters for standard curve calculations and protocol management for immunoassays.

Cut-off protocols

Details parameters and settings specific to cut-off measurement protocols.

Data storage

Specifies the instrument's capacity for storing measurement data.

Interfaces

Lists the available interfaces, notably the RS 232 port for computer connection.

Ambient temperature

Specifies the operating and storage temperature ranges for the instrument.

16. Flow diagram of the measurement procedure

Schematic representation of the measurement procedure

Provides a table detailing the measurement steps, user actions, instrument actions, and displays.

Appendix

A.1 Mathematical Basis

Explains the mathematical principles behind data reduction and curve fitting for LIA assays.

A.2 Data Acquisition of the Lumat LB 9507 via the Serial Interface (I/O-Port) and Saving this Data in a MS-DOS ASCII-File

Details how to acquire and save measurement data to an ASCII file using terminal software.

LUMAT LB 9507 User Manual

Table of Contents