LUMAT LB 9507 - 9.1 Creating Protocols for Dual-LuciferaseTM Assay

LB 9507 Chapter 9: Dual-Luciferase
TM
Reporter Gene Assay
9-3
9.1 Creating Protocols for Dual-Luciferase
TM
Assay
9.1.1 With 2 injectors installed
D1
READY
MEASURE PROTOCOL -OTHERS-
Select the <PROTOCOL> menu by pressing the button located
directly below. The following display appears:
D2
PROTOCOL
MASTER CURVE CREATE CHANGE
The <MASTER CURVE> option can be used only when a
“quantitative protocol” without a standard series has been created
and stored (see Chapter 7.2). It is not relevant to raw data
measurements.
Select <CHANGE> to open an existing protocol by entering its
protocol number. You can then edit it.
To create a new protocol, select <CREATE>. The following
display appears:
D3
06: PROTOCOL NAME DLR-LYSATE1
The next free number is displayed for the new protocol (in this
example “06:”). Enter a name for this protocol using the
alphanumeric keyboard. You should use a name which specifies
the protocol type and other important features, so that it is easy to
identify later. For example “DLR-LYSATE1”.
Confirm your entry with <enter>.
D4
06: PROTOCOL TYPE
RAW DATA CUTOFF -OTHERS-