Chapter 9: Dual-Luciferase
TM
Reporter Gene Assay LB 9507
9-4
D5
06: PROTOCOL TYPE
QUANT TUP/FTI -OTHERS-
D6
06: PROTOCOL TYPE
DL-ASSAY ------ -OTHERS-
Select <DL-ASSAY>. Now you have to enter the parameters
which are the same for all protocol types:
D7
06: OPERATOR ID.
Enter the user name and press <enter>.
D8
06: INJECTOR VOLUME 1 [µl] 0
(0 – 300) LAST DISPLAY
Changing or confirmation of the displayed injector volume.
Displayed are the standard settings for injector 1 entered in the
<INSTRUMENT PARAMETERS> menu. These settings can be
changed for this specific protocol.
Setting "0" means the respective injector is disabled.
Range: 25 µL - 300 µL in 5 µL steps.
If the user enters the injector volume not within the 5 µL step
range, the system rounds up or down. Values out of the range are
adjusted to the limits (25 µL or 300 µL).
Example: When entering 43 µL the system rounds up to 45 µL,
when entering 102 µL the system rounds down to 100 µL.
Press <enter> to use the response displayed in the upper-right
corner of the LCD.
D9
06: MEASURE BACKGROUND NO
YES NO LAST DISPLAY
Select here if the background rate is to be measured prior to the
injections and the actual measurement of each tube.
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