LB 9507 Chapter 9: Dual-Luciferase
TM
Reporter Gene Assay
9-5
If you select <NO>, display D13 appears or, when no injector is
selected, D14.
<YES> takes you to the next display:
D10
06: MEASURING TIME BKG [s] 0.5
(0.1 - 200.0) LAST DISPLAY
Enter between 0.1 and 200.0 seconds for the background
measurement and press <enter>. Reasonable times start at half
a second, allowing statistical variations to be compensated for.
Otherwise, the background values may be too high or too low
which either distorts the result of the measurement or leads to an
exceeding of the threshold.
Remember that the background effect is measured in order to
obtain an alarm threshold for background rates that are too high.
D11
06: AUTOMATIC BKG SUBTRACTION NO
YES NO LAST DISPLAY
Here you can select whether the background effect is to be
subtracted automatically from the measured results. Remember
that the statistical significance of the measured values is
dependent upon the measuring time.
D12
06: MAX. BACKGROUND [RLU/s] 50
(0 = NONE) LAST DISPLAY
Here can you define the limit value for the background. If this
threshold is exceeded, a direction is displayed and printed out
(BKG).
Entering “0” means “no background threshold“.
As soon as you confirm the entry with <enter> you move to the
next display.
D13
06: DELAY TIME INJ 1 / MEAS 1 [s] 0.0
(0.0 – 300.0) LAST DISPLAY
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