LUMAT LB 9507 - 7. Quantitative Measurements (LIA...); 7.1 Introduction

LB 9507 Chapter 7: Quantitative Measurements (LIA...)
7-1
7. Quantitative Measurements (LIA...)
7.1 Introduction
This type of measurement is used for reagent kit specific
measurements to quantify analyte concentrations (LIA, ILMA,
etc.). Three measurement strategies are available:
1. Measurement with complete standard series
Known standard concentrations are measured before the
unknown samples. After transformation of the calibration curve
(either log-log or logit-log) interpolations between standards and
unknown samples take place. The curve fit is based on a cubic
spline function and is excellently suited to LIAs and ILMAs. If
necessary, the curve can be smoothed either automatically or
upon the user’s instruction.
2. Measurement with master curve
This measurement type uses 2 calibrators instead of standard
concentrations, which are provided as samples by the assay kit
manufacturer. Once this protocol has been created, the remaining
value pairs (RLU/conc) of a standard curve are entered in the
submenu <MASTER CURVE> and are used as a logit-log
transformed master curve to analyse the samples. The value
pairs are included with the respective batch of reagent kits.
The displays are numbered consecutively L1, L2 ...LX for clarity.
Fig. 7-1 is a flow chart of the protocol setup procedure, entry of
the master curve and start of the measurement.