LUMAT LB 9507 - 5. Raw Data Measurements; 5.1 Introduction

LB 9507 Chapter 5: Raw Data Measurements
5-1
5. Raw Data Measurements
5.1 Introduction
In the Raw data measurement mode the quantity of light
measured over a user-defined measuring time is measured in
RLUs for each sample. You have the option of printing out a high
resolution (up to 2000 data point) curve and/or up to 50 data
points with measurement time for each sample, which enables
you to analyze the kinetic behaviour of the light emission of a
sample.
Replicate groups can also be defined for which the average value
and the variation coefficient are calculated and printed out.
This measurement mode, which allows up to 2 injections per
sample, is used mainly for ATP measurements and for
measurement of luminescence reporter gene assays.
There are two ways of starting raw data measurement (see Fig.
5-1):
1. Create and save protocols for routine measurements in the
<PROTOCOL> menu (max. 40). With each of these protocols
you can later start as many measurements as you want in the
<MEASURE> menu.
2. Running a raw data direct measurement.
Select the option < MEASURE > from the <DIRECT ENTRY>
menu to enter the measurement parameters. Then start the
measurement.
In both cases the parameters you are asked to enter are the
same.
The displays are numbered consecutively from R1... R34 for
clarity.
The following illustration shows a flow chart of the protocol setup
procedure and measurement start for routine and direct
measurements.