LB 9507 Chapter 9: Dual-Luciferase
TM
Reporter Gene Assay
9-11
9.1.3 Without injectors
The instructions here describe how to create a protocol when no
injectors are installed. The reagents have to be injected manually
between the measurements. The sample tubes are turned to the
loading position and the injection can start.
D1
READY
MEASURE PROTOCOL -OTHERS-
Select the <PROTOCOL> menu by pressing the respective
button. Then follows:
D2
PROTOCOL
MASTER CURVE CREATE CHANGE
The <MASTER CURVE> option can be used only when a
“quantitative protocol” without a standard series has been created
and stored (see chapter 7.2). This option is not relevant to Dual-
Luciferase™ assay.
If you press the button below <CHANGE> you can open an
existing protocol by entering its protocol number, and edit it. To
create a new protocol, press the button below <CREATE>. When
you do this the following display appears:
D3
06: PROTOCOL NAME DLR-LYSATE1
The next free number is displayed for the new protocol (in our
example “06:”). Enter a name for this protocol using the
alphanumeric keyboard. You should use a name which specifies
the protocol type and other important features, so that it is easy to
identify later. For example “DLR-LYSATE1”.
Confirm your entry with <enter>.
D4
06: PROTOCOL TYPE
RAW DATA CUTOFF -OTHERS-
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