LUMAT LB 9507 - Page 150

Chapter 9: Dual-Luciferase
TM
Reporter Gene Assay LB 9507
9-12
D5
06: PROTOCOL TYPE
QUANT TUP/FTI -OTHERS-
D6
06: PROTOCOL TYPE
DL-ASSAY -OTHERS-
Select <DL-ASSAY>. Now you have to enter the parameters
which are the same for all protocol types:
D7
06: OPERATOR ID
Type the user name and press <enter>.
D8
06: MEASURING TIME 1 [s] 2.0
(0.1 - 200.0) LAST DISPLAY
Enter the desired measuring time (0.1 to 200.0 seconds) for the
first measurement for each tube and press <enter>.
D9
06: MEASURING TIME 2 [s] 2.0
(0.1 - 200.0) LAST DISPLAY
Enter the desired measuring time (0.1 to 200.0 seconds) for each
tube and press <enter>.
D10
06: NUMBER OF REPLICATES 2
(1 - 10) LAST DISPLAY
Enter the number of replicates and press <enter>. You can define
a maximum of 10 sample tubes as replicates whose measured
values are averaged. “1” means 1 tube, i.e. no replicates.