LUMAT LB 9507 - With 1 Injector Installed

Chapter 9: Dual-Luciferase
TM
Reporter Gene Assay LB 9507
9-8
9.1.2 With 1 injector installed
The instructions here describe how to create a protocol when only
one injector is installed. This measurement mode can be used
only when the starter reagent is manually injected. The Stop and
Glo™ reagent is injected with the internal injector.
D1
READY
MEASURE PROTOCOL -OTHERS-
Select the <PROTOCOL> menu by pressing the respective
button. Then follows:
D2
PROTOCOL
MASTER CURVE CREATE CHANGE
The <MASTER CURVE> option can be used only when a
“quantitative protocol” without a standard series has been created
and stored (see chapter 7.2). This option is not relevant to Dual-
Luciferase™ assay.
If you press the button below <CHANGE> you can open an
existing protocol by entering its protocol number, and edit it. To
create a new protocol, press the button below <CREATE>. The
following display is shown:
D3
06: PROTOCOL NAME DLR-LYSATE1
The next free number is displayed for the new protocol (in our
example “06:”). Enter a name for this protocol using the
alphanumeric keyboard. You should use a name which specifies
the protocol type and other important features, so that it is easy to
identify later. For example “DLR-LYSATE1”.
Confirm your entry with <enter>.
D4
06: PROTOCOL TYPE
RAW DATA CUTOFF -OTHERS-