LB 9507 Chapter 9: Dual-Luciferase
TM
Reporter Gene Assay
9-9
D5
06: PROTOCOL TYPE
QUANT TUP/FTI -OTHERS-
D6
06: PROTOCOL TYPE
DL-ASSAY -OTHERS-
Select <DL-ASSAY>. Now you have to enter the parameters
which are the same for all protocol types:
D7
06: OPERATOR ID
Type the user name and press <enter>.
D9
06: INJECTOR VOLUME 1 [µl] 0
(0 – 300) LAST DISPLAY
Changing or confirmation of the displayed injector volume for the
injection 1. Displayed are the standard settings for injector 1
entered in the <INSTRUMENT PARAMETERS> menu. These
settings can be changed for this specific protocol.
Setting "0" means the respective injector is disabled.
Range: 25 µL - 300 µL in 5 µL steps.
If the user enters the injector volume not within the 5 µL step
range, the system rounds up or down. Values out of the range are
adjusted to the limits (25 µL or 300 µL).
Example: When entering 43 µL the system rounds up to 45 µL,
when entering 102 µL the system rounds down to 100 µL.
Press <enter> to use the response displayed in the upper-right
corner of the LCD.
D10
06: DELAY INJ.1/MEAS.2 [s] 0.0
(0.0 - 300 s) LAST DISPLAY
Enter the delay between the injection and the start of the
measurement in a range from 0.0 to 300 seconds and press
<enter>.
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