LUMAT LB 9507 - Page 148

Chapter 9: Dual-Luciferase
TM
Reporter Gene Assay LB 9507
9-10
D11
06: MEASURING TIME 1 [s] 2.0
(0.1 - 200.0) LAST DISPLAY
Enter the desired measuring time (0.1 to 200.0 seconds) for the
first measurement for each tube and press <enter>.
D12
06: MEASURING TIME 2 [s] 2.0
(0.1 - 200.0) LAST DISPLAY
Enter the desired measuring time (0.1 to 200.0 seconds) for each
tube and press <enter>.
D13
06: NUMBER OF REPLICATES 2
(1 - 10) LAST DISPLAY
Enter the number of replicates and press <enter>. You can define
a maximum of 10 sample tubes as replicates whose measured
values are averaged. “1” means 1 tube, i.e. no replicates.
D14
06: NORMALIZED RESPONSE M1/M2
M1/M2 M2/M1 LAST DISPLAY
Normalized response gives the quotient of both measured values:
M1/M2= RLU/s Firefly luciferase
RLU/s Renilla luciferase
M2/M1= RLU/s Renilla luciferase
RLU/s Firefly luciferase
D15
06: INPUT ALL CORRECT ? YES
YES NO LAST DISPLAY
If you select <YES> the entered parameters are stored and the
program returns to the main menu (D1). <NO> takes you back to
the 1st display of this protocol (D7).
Note: The protocol is not stored if you exit this protocol sequence
via <exit>!