Contents LB 9507
ii
5.1 Introduction 5-1
5.2 Creating Raw Data Protocols 5-3
5.3 Editing/Deleting Protocols 5-9
5.4 Performing Raw Data Measurements 5-11
6. CUT-OFF MEASUREMENTS 6-1
6.1 Introduction 6-1
6.2 Creating CUT-OFF Protocols 6-4
6.3 Editing/Deleting Protocols 6-12
6.4 Running Cut-off Measurements 6-14
7. QUANTITATIVE MEASUREMENTS (LIA...) 7-1
7.1 Introduction 7-1
7.2 Setting Up Quantitative Protocols 7-3
7.3 Editing/Deleting Protocols 7-12
7.4 Entering a Master Curve 7-14
7.5 Performing Quantitative Measurements 7-17
7.5.1 Measurement with a Complete Standard Curve 7-17
7.5.2 Measurements with Master Curve 7-25
8. T-UPTAKE/FTI-MEASUREMENTS 8-1
8.1 Introduction 8-1
8.2 Creating T-uptake/FTI protocols 8-3
8.3 Editing/Deleting protocols 8-12
8.4 Performing T-uptake/FTI Measurements 8-14
9. DUAL-LUCIFERASE
TM
REPORTER GENE ASSAY 9-1
9.1 Creating Protocols for Dual-Luciferase
TM
Assay 9-3
9.1.1 With 2 injectors installed 9-3
9.1.2 With 1 injector installed 9-8
9.1.3 Without injectors 9-11
9.2 Editing/Deleting Protocols 9-14
9.3 Performing Dual-Luciferase
TM
Reporter Gene Assay
Measurements 9-16
10. KINETIC AND REPEATED MEASUREMENTS 10-1
10.1 Kinetic Option in Raw Data Protocol 10-1
10.2 Repeated Measurements 10-2
10.2.1 Creating Repeated Protocols 10-4
10.2.2 Editing/Deleting Protocols 10-9
10.2.3 Performing Repeated Measurements 10-11
10.3 Ratemeter 10-14
11. OPERATOR FUNCTIONS 11-1
11.1 <REAGENT> Function 11-2
11.1.1 Loading reagents 11-3
11.1.2 Refreshing reagents 11-4
11.1.3 Washing of the injector system 11-5
11.1.4 Removing reagents 11-7
11.2 Performance test 11-8
11.3 Manual Data Entry 11-9
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