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8.1.2 Five-part diff measurement
The innovative optical detection system is covered by two patents pending. This technology (called OCHF for
Optical Cytometer Hydrofocus Free) is based on an unique and innovative concept of an active sample flow
and a passive sheath.
The sample flow is introduced in the flow cell under pressure and the sheath is only dedicated to maintain it.
This principle enables to introduce a large quantity of sample and to use a great dilution rate (which allows
doing the Hemoglobin measurement with the same dilution).
The main advantages are:
- High level of reliability of the optical adjustment.
- Only two measurement axes for five parameters.
- High resolution matrix.
- Only one passive sheath with low reagent consumption.
- No hydro focusing system.
- Low level of contamination between two measurements.
- Very low cost flow cell (injected plastic).
- Sample flow
- Optical window
- Light beam (455 nm wavelength)
- Passive sheath
- Injector
- Sample WBC dilution
For each cell throwing the optical detection area, two pulses are
generated, one for the Axis Loss Light (ALL) measurement and one
for the Forward Side SCatter (FSC) measurement.
The result of those two axes of measurement is the high definition
matrix that enables to identify five WBC populations.
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