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CIRAS-3 Operation Manual V. 1.09 92 support@ppsystems.com

phiPSII-SP (ϕPSII-Single-Pulse). If selected, a single, saturating pulse will be

applied to the light-adapted leaf sample. The single pulse duration and

intensity can be set in the lower part of the screen under “Saturating Light”.

phiPSII-MP (ϕPSII-Multi-Pulse). If selected, multiple, saturating pulses will be

applied to the light-adapted leaf sample. The Multi-Pulse duration and

intensity settings (up to 5 steps) can be set in the lower part of the screen

under “Saturating Light”.

ϕPSII. If this is selected, a saturating pulse will be applied to the light-adapted

leaf sample after a photosynthesis measurement has been recorded.

phiPSII-FoP (ϕPSII-Fo’). If this is selected, a saturating pulse will be applied

to the light-adapted leaf sample followed by illumination using the far-red light

for measurement of Fo’ after a photosynthesis measurement has been

recorded.

Fs. If this is selected, the steady state fluorescence will be recorded after a

photosynthesis measurement is recorded.

Enter the estimated Total Manual or Timed Repeated Records for the

measurement session you will conduct. For example, if you will make

measurements in Manual recording mode, and you know that you will apply 5

different light levels for which you would like to record chlorophyll fluorescence

once with far-red light application immediately following each saturating pulse,

select ϕPSII-Fo’ after every 1 records, and Total Manual or Timed

Repeated Records=5. If using a Response Curve to measure fluorescence,

Repeated Fluorescence will be used in conjunction with Records per Level in

the response curve.

To make only a single Initial measurement (for example, to dark-adapt and

perform Fv/Fm on multiple leaf samples) set Total Manual or Timed

Repeated Records=0.

Concluding

Fluorescence

Measurement

This defines the final measurement to be recorded after the last

photosynthesis measurement.

None. If this is selected, there will be no concluding chlorophyll fluorescence

measurement and the sequence will terminate after the last photosynthesis

measurement.

PP Systems CIRAS-3 User Manual

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