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A Accessories and platepads
Setting up thermal and shaking stations (Inheco controller)
Bravo Platform User Guide
As the figure shows, the height difference between the adapter top (1) and the plate
nest top (2) enables a skirted microplate to nest in the station. The well bottoms must
sit directly on the adapter top to enable an efficient temperature transfer. However, the
teachpoint must account for this height difference to ensure that the Bravo robot grips
the microplate at the correct position and performs pipetting tasks at the correct
height.
If your microplate does not sit in the plate nest as the figure shows, contact Agilent
Automation Solutions Technical Support for guidance on whether your microplate
requires a custom plate nest and adapter.
Use the following procedure only on a Bravo Platform with a disposable-tip head.
To set the teachpoint for a generic Peltier Thermal Station or Heating Shaking Station:
1 Place the microplate securely in the plate nest of the thermal station. Verify that
the microplate sits in the plate nest so that the well bottoms are resting on the
adapter and the skirt rests in the plate nest bottom surface. Remove the
microplate.
2 Using calipers, measure the height difference between the adapter top (1) and the
plate nest top (2) on all four sides of the plate nest interior. Make a note of the
smallest number. Later in the procedure, you will add this value to the z-axis value
for the teachpoint.
3 Place the teach plate in the plate nest.
Note: Alternatively, you can use a 384-well microplate instead of the teach plate
to set the teachpoint xy coordinates. In this case, you set the xy coordinates
based on the center of the A1 well quadrant, as the following figure shows.
4 In Bravo Diagnostics, click the Profiles tab, and initialize the desired profile.
5 In Bravo Diagnostics, click the Processes tab, select a tip box, and perform a Tips On
command. For details, see “Performing a task using Bravo Diagnostics” on
page 100.
Using the wrong tip for teaching can result in a head crash. Make sure the disposable
tip is an Agilent tip and is the same as the Teaching tip type specified in the profile.
6 In Bravo Diagnostics, click the Jog/Teach tab, and set the initial teachpoint as
follows:
a Select the deck location of the station from the Location list.
b Use the controls to jog the head so that the A1 pipette tip is directly over the
crosshairs with paper-thin clearance. For details, see “Setting the first
teachpoint” on page 36.
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