2 Setting up the Bravo Platform
Setting teachpoints
36 Bravo Platform User Guide
Workflow for a tip box location
Pressing pipette tips on the liquid-handling head requires a high degree of precision in
the positioning of the head relative to the tip box, especially for 384-well tip boxes. Use
the following workflow for deck locations where you plan to place either a tip box.
Setting the first teachpoint
If you are using a:
• Disposable-tip head, place the teaching tip type that you specified in the profile on
the A1 barrel or probe to set the teachpoint.
• Bravo 96AM Head, see the AssayMAP Bravo Platform Installation Guide.
• Pin tool or fixed-tip head, set the teachpoint according to the A1 needle.
To set the first teachpoint:
1 In Bravo Diagnostics, click the Profiles tab, and initialize the desired profile.
2 Disposable-tip heads. Place a pipette tip firmly on the head barrel that corresponds
to the A1 well of the labware you are using.
Step For this task... See...
1 Determine the platepad requirements for the tip box location:
• Standard Bravo Platform
– ST tip box. Use an Alignment Station instead of a
standard platepad at the deck locations where you
perform tip box operations. The Alignment Station helps
provide greater tip-loading precision.
“Installing and configuring a
platepad or Alignment Station”
on page 140
– LT t ip b o x. If using a 250-µL tip box, you can use a
standard platepad or an Alignment Station.
Note: If using a 200-µL tip box, use a standard
platepad and an LT insert for tip loading.
–
– Nested tip box. Install and configure the Nested Rack
Insert.
“Installing and setting up a
Nested Rack Insert” on
page 147
• SRT Bravo Platform
– ST tip box. Use an ST tip loading station.
– 250-µL tip box. Install the 250-µL platepad and configure
the deck location.
“(Bravo SRT only) Setting up an
LT tip box location” on page 172
2 Set the teachpoint for the location. “Setting the first teachpoint” on
page 36
3 ST tip box locations. Verify the labware definition for the tip box. “Verifying teachpoints for
disposable-tip heads” on
page 39
4 Verify the teachpoint. If tip-box operations are not precise
enough, repeat this step.
“Verifying teachpoints for
disposable-tip heads” on
page 39