Amsco 110LS - 3 TECHNIQUES OF STERILIZATION; 3.1 Recommended Sterilization Variables

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Techniques of Sterilization Operator Manual 387366-389
TECHNIQUES OF STERILIZATION
3.1 Recommended
Sterilization
Variables
3.1.1 Prevacuum Cycle The Prevacuum cycle is recommended to process heat- and moisture-
stable goods [except liquids] which are capable of being sterilized
with steam. This cycle can also be used to decontaminate wastes,
including wastes containing liquids, provided the materials are
properly contained.
Refer to Table 3-1 for recommended Prevacuum cycle parameters.
* Minimum sterilize times are based on obtaining a 10
6
Sterility Assurance Level
(SAL) with standard test loads. Specific loads may require different sterilize times
to achieve this level of sterility, or may require a different SAL.
3.1.2 Gravity Cycle Refer to Table 3-2 for the type of items which can be processed in a
Gravity cycle and the recommended parameters.
Table 3-1. Prevacuum Cycle Parameters
Temperature
Pressure Point
Psig (psia)
Minimum Recommended
Sterilize Time*
Minutes at Temperature
121°C (250°F) 12-14 (27-29) 15
132°C (270°F) 26-28 (40-42) 4
Table 3-2. Gravity Cycle Parameters
Items
Minimum
Recommended
Sterilize Time at
121°C (250°F)
(minutes)
Minimum
Recommended
Sterilize Time at
132°C (270°F)
(minutes)
Dry Time
(minutes)
Glassware
Empty, inverted,
vented*
15 Min. 3 Min. 0 Min.
†
Instruments
metal combined
with suture,
tubing or other
porous materials
(unwrapped)
20 Min. 10 Min. 0 Min.
†
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