Amsco 110LS - 3.3 Recommendations for the Sterilization Process

3-4
387366-389 Operator Manual Techniques of Sterilization
3.2.3 Vacuum Leak Test
After running a Leak Test cycle, a value or leak rate is printed on
tape. The value helps in establishing a trend over a period of time
and can indicate when the integrity of the system is beginning to
deteriorate (i.e., allowing air to enter the system).
Operators and maintenance personnel run a leak test daily or weekly
to monitor the system air-tight integrity and make repairs as needed.
A leak rate of greater than 1 mmHg per minute indicates a problem
with the sterilizer that must be addressed.
3.3 Recommendations
for the Sterilization
Process
Saturated steam is a well controlled, reliable method for processing
items which can withstand the temperatures and pressures
associated with steam sterilization. The requirements for achieving
reproducible results are well known by many users, but are not
always understood by all users.
The condition most likely to result in sterilization problems is a failure
to remove all of the air from the items being processed. For example,
placing an empty beaker or bowl in an upright position in a gravity
displacement sterilizer may result in the object not being sterilized,
or may require exceptionally long sterilization times. This problem is
caused by air trapped in the object; air has almost twice the density
as does saturated steam under the same conditions. Thus, the air
remains in the bottom of the container while steam forms a stable
layer above it. (Similar to oil forming a stable layer over water.) As
long as there is no mechanism for actively mixing air and steam, the
bottom of the container is only exposed to dry heat, which is not an
effective sterilization method at the time and temperatures typically
used in steam processes.
The best method for enhancing sterilization of solid-bottom
containers in gravity displacement cycles is to orient all objects in a
position which would allow water to flow out. When steam enters the
chamber, it tends to layer over trapped air. If, however, an object is
oriented so air is not trapped, but can flow out; air flows out to be
replaced by steam. Steam can now reach all surfaces and effect
sterilization.
WARNING – STERILITY
ASSURANCE HAZARD: Load
sterility may be compromised
if the biological or chemical
indicator, or vacuum leak test
indicates a potential problem.
If these indicators show a
potential problem, refer the
situation to a qualified service
technician before using the
sterilizer further.
!