3-2
387366-389 Operator Manual Techniques of Sterilization
* If items which can trap air must be sterilized upright, they should be sterilized in
a prevacuum cycle.
† Goods will be wet when removed from sterilizer.
‡ Dry time can vary for wrapped goods depending on pack density, weight
of goods, pack preparation techniques including type of wrapping material
used, and sterilizer loading procedures.
3.1.3 Liquid Cycle Refer to Table 3-3 for recommended Liquid cycle parameters. The
recommended times indicated in Table 3-3 assume the use of
vented bottles or Erlenmeyer flasks. The minimum sterilization time
includes the time required to bring the solution up to the sterilization
temperature plus the time required to achieve sterilization.
NOTE: Use load probes and F
o
option to optimize cycle times.
* Minimum sterilize times are based on obtaining a 10
6
Sterility Assurance Level
(SAL) with standard test loads. Specific labs may require different sterilize times
to achieve this level of sterility, or may require a different SAL.
Hard Goods
Unwrapped
15 Min. 3 Min.
0 Min.
†
Hard Goods
Wrapped in
muslin or
equivalent
30 Min. 15 Min. 30 Min.
‡
Table 3-2. Gravity Cycle Parameters (Continued)
Items
Minimum
Recommended
Sterilize Time at
121°C (250°F)
(minutes)
Minimum
Recommended
Sterilize Time at
132°C (270°F)
(minutes)
Dry Time
(minutes)
Table 3-3. Liquid Cycle Parameters – No Load Probes
Volume of Liquid in One
Container (mL)
Minimum Recommended
Sterilize Time at 121°C (250°F)
(minutes)*
75 25
250 30
500 40
1000 45
1500 50
2000 55
>2000 55+10 min/L
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