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Amsco 110LS
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6-33
Sterilizer Operation Operator Manual 387366-389
The ATF 1 (Alternating Tangential Flow 1) cycle is a method that
can be used to sterilize XCell™ ATF System.
1
The ATF 1 cycle can
also be used for other purposes requiring sterilization features of this
cycle.
Run a ATF 1 CYCLE by pressing ATF 1 button from one of operating
mode screens (S
ECTION 5.2, OPERATING MODES). The following screen
appears (values are examples only):
Press LEFT ARROW button to cancel selected cycle and return user
to operating mode screen.
Press START CYCLE to start ATF 1 Cycle.
NOTE: If CLOSE DOOR(S) button is shown, close door(s).
Refer to S
ECTION 5.3, STATUS BUTTONS, for description of buttons.
JACKET CHARGE PHASE: Jacket charge phase continues until
jacket temperature is greater than set sterilize temperature (example
121.0°C (250.0°F)). If separate steam selected, jacket charge phase
continues until jacket temperature is greater than set jacket
temperature, for example: 15°C (239°F).
ACTIVATE SEAL PHASE: Door gasket seals with steam until door
seal switch is closed. An additional 20 seconds elapses after seal
switch is closed before phase is complete.
PREHEAT: The product in chamber is heated to 60°C (140°F) for 90
minutes using jacket heat only (no direct steam). The chamber
temperature is used to determine 60°C (140°F) temperature.
PURGE PHASE: Steam enters chamber from jacket (or direct with
separate steam) to control pressure at 6 psig (0.41 bar). Fast exhaust
aids in removing air from chamber. The duration is 5 minutes.
PULSE EXHAUST PHASE: Chamber exhaust to 4.0 psig (0.28 bar)
with an exhaust rate of 1 psi per minute. Jacket temperature is
controlled to maintain approximately 122.5°C (253.0°F) in jacket. Units
with separate steam control jacket at 115°C (239.0°F).
1. XCell™ is a registered trademark of Repligen Corporation.

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