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387366-389 Operator Manual Sterilizer Operation
The ATF 2 (Alternating Tangential Flow 2) cycle is one method that
can be used to sterilize XCell™ ATF System.
1
This cycle can also be
used for other purposes that require sterilize features of this cycle.
Run a ATF 2 CYCLE by pressing ATF 2 button from one of operating
mode screens (S
ECTION 5.2, OPERATING MODES). The following screen
appears (values are examples only):
Press LEFT ARROW button to cancel selected cycle and return user
to operating mode screen.
Press START CYCLE to start ATF 2 Cycle.
NOTE: If CLOSE DOOR(S) button is shown, close door(s).Refer to
S
ECTION 5.3, STATUS BUTTONS, for description of buttons.
JACKET CHARGE PHASE: Jacket charge phase continues until
jacket temperature is greater than set sterilize temperature (example
121.0°C (250.0°F)). If separate steam selected, jacket charge phase
continues until jacket temperature is greater than set jacket
temperature, for example: 115°C (239°F).
ACTIVATE SEAL PHASE: Door gasket seals with steam until door
seal switch is closed. An additional 20 seconds elapses, after seal
switch is closed, before phase is complete.
PULSE EVACUATE PHASE: Chamber evacuates to 25.0 inHg (-0.84
bar) with an exhaust rate of 1 psi per minute. Jacket temperature is
controlled to maintain approximately 122.5°C (253.0°F) in jacket. Units
with separate steam will control jacket at 115°C (239.0°F).
PULSE VACUUM HOLD: Chamber holds at 25.0 inHg for five minutes.
Jacket temperature is controlled to maintain approximately 122.5°C
(253.0°F) in jacket. Units with separate steam will control jacket at
115°C (239.0°F).
1. XCell™ is a registered trademark of Repligen Corporation.
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