Chapterl 1: System Description LB 9507
1-6
The program runs through the individual steps for each sample
in accordance with the settings in the measurement protocol:
background measurement, injections, measurement.
Upon completion of the background measurement, the pumps are
activated one after the other. If required, pump # 1 injects a
variable amount of the first reagent into the sample tube. After a
defined delay, pump # 2 also injects a variable amount (which
may differ from the 1st injection) of the second reagent into the
sample tube. Of course, measurements without or with only one
injection are also possible.
The injection of the 2nd reagent typically initiates the chemical
reaction resulting in the emission of light photons. The number of
photons that are emitted during this reaction is indirectly related to
the analyte concentration in the sample.
The photons hitting the cathode of the photomultiplier are
transformed into electrons and amplified as single pulses. A
threshold discriminator suppresses the noise signals of the
photomultiplier. The computer counts the pulses and supplies the
integral result of the light signal (photon counting method).
Every measurement step is automatically documented on the
thermal printer together with the measurement results obtained.
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