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Cedex HiRes Analyzer Operator’s Guide
D
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Administrator Functions
Adjustment of the Image Analysis using the Live Operator
7.5 Adjustment of the Image Analysis using the Live Operator
The Cell Type (operator) selected for a measurement takes care of the image evaluation. For various application
scenarios, Roche Diagnostics GmbH has put in three pre-settings that are adapted to a wide cell size spectrum:
Min. Size for small cells with a diameter of about 6 µm, e.g. lymphocytes, default sedimentation time is
90 seconds.
Std. Size for normal-sized cells with a diameter of about 12 µm, e.g. HB58, default sedimentation time is
45 seconds.
Max. Size for large cells of more than 16 µm, e.g. K562, default sedimentation time is 45 seconds.
The Cell Types may have slightly different names if the system has been upgraded from some previous
version to Software version 2.2. In the case of a system that has been upgraded to version 2.2, the default
sedimentation time for all Cell Types may be 90 seconds. Sedimentation time can be adjusted via the Cell
Type List window as described below.
Individual adjustments of the operator can be helpful or necessary for cell cultures that, for instance, have an
unusual optical appearance or for a counting strategy that deviates from the Cedex HiRes Software 2.2 analysis
(e.g. counting aggregates differently).
Adjustment of the operator is referred to as creating a new Cell Type. A new Cell Type can be created or an
already existing Cell Type can be re-adjusted. This can be done with the help of ten parameters that infl uence
the way in which an image is evaluated.
Each of these parameters directly infl uences the image processing strategy and, hence, the measurement results.
In the available software version, an algorithm with the three pre-settings mentioned above is available. The
parameters of the three factory installed pre-settings are overwritable.
For data safety reasons the parameters of the three factory installed pre-settings should not be altered. Since
original settings are overwritable, caution ought to be advised for all authorized users.
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