Roche Cedex HiRes - Editing an Existing Cell Type; Viewing an Existing Cell Type

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Software
D
D
Administrator Functions
Adjustment of the Image Analysis using the Live Operator
7.5.4. Editing an Existing Cell Type
An existing Cell Type can be modifi ed. However, it is NOT advisable for data safety reasons to modify an
existing Cell Type that has been used for measurements. A new Cell Type based on an existing Cell Type
should be created instead.
Follow the instructions below to modify an existing Cell Type (not advisable!):
1
Select the Cell Type to be edited from the list of Cell Types at the top of the Cell Type List window and then
click on the Edit button. The Cell Type window will open.
2
Modify the following information if desired:
 The name of the Cell Type
 Sample preparation procedure (FMV is currently the only option)
 Focus Offset
 Sedimentation Duration
3
Make sure that the IR Operator is set to CXHiResImageOperator.
4
Click on the Live Operator button in order to modify other parameters within the Live Operator window for
that Cell Type (see “
Working with the Live Operator”).
5
In the Data Source area, select the Work Area, followed by the Reactor ID, Sample ID and Data Set
of the measurement for which the image analysis should be improved. The parameters for the loaded
measurement are preset.
6
Select an image of the measurement in the Image ID section. This image will be loaded and displayed in
the window in the upper right-hand corner with the living cells marked with a green circle and the dead cells
marked with a red circle.
7
Make the desired modifi cations on a single parameter fi rst (see “Description of the Individual Operator
Parameters
”). As soon as a parameter has been modifi ed, data reprocessing is automatic. The image display
then shows the reprocessed image.
8
When an optimized adjustment of the Operator parameters has been found, click on Save to confi rm the
modifi cations.
9
Click on Cancel to close the window without saving any changes.
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7.5.5. Viewing an Existing Cell Type
Select the Cell Type to be viewed from the list of Cell Types at the top of the Cell Type List window and then
click on the View button. The Cell Type window will open in which the information for focus point (Focus
Offset) and sedimentation time (Sedimentation Duration) can be viewed. Click on the Live Operator button
in order to view other parameters within the Live Operator window for that Cell Type (see “Description of the
Individual Operator Parameters”).