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Cedex HiRes Analyzer Operator’s Guide
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3.2 Defi ning the Measurement and Analysis (Process) Parameters
The measurement and analysis parameters are defi ned in the Processing Parameters area of the Measurement
window. In principle, all routine measurements can be carried out using the standardized default settings that
are factory set. To change the settings, follow the instructions below:
1
Select the sample port type in the Sample Port drop-down list:
Multi or Auto: this option will only appear on systems with Multi Samplers.
Single: this option will only appear on systems with a single sample port.
Remote: This option is not yet available.
2
Manually enter the cup position for the sample in the Cup Position window if there is a Multi Sampler (see
“
Working with the Multi Sampler”).
In the case of single measurements run via the Measurement window on a Cedex HiRes System with
a Multi Sampler attached, the software will auto matically incrementally increase the sample cup position
number with each new measurement. The cup position can also be fi xed (see “
Turning off automatic
incrementation of sample cup position
”).
3
Select the Cell Type that optimally recognizes your cells from the Cell Type drop-down list. (see “Adjustment
of the Image Analysis using the Live Operator
”). The following Cell Types are available by default:
Min. Size for small cells with a diameter of about 6 µm, e.g., lymphocytes, default sedimentation time is
90 seconds.
Std. Size for normal sized cells with a diameter of about 12 µm, e.g., HB58, default sedimentation time is
45 seconds.
Max. Size for large cells of more than 16 µm, e.g., K562, default sedimentation time is 45 seconds.
If a new Cell Type has been created, it can also be selected from the same Cell Type drop-down list. See
“
Adjustment of the Image Analysis using the Live Operator” for more detailed information about creating new
Cell Types.
The 45 second sedimentation time set by default for the Std. Size and Max. Size may not allow enough
time for a prescan to be performed. In some cases of slow computer systems, the prescan may require
slightly more time than the default sedimentation time. If this occurs, a message “Warning: sedimentation
time was exceeded” will appear in the main Cedex Control Center window. However, there will be no
effect on the measurement procedure other than a slightly longer sedimentation time. The sedimentation
time can be increased for the Std. Size and Max. Size, if desired, in order ensure a sedimentation
time that is longer than the prescan time (see “
Editing an Existing Cell Type”). Alternatively, the Prescan
function can be turned off (see “
Turning off the Flow Chamber Prescan”).
The Cell Types may have somewhat different names and sedimentation times if the system has been
upgraded from some previous version to Software version 2.2. In the case of a system that has been
upgraded to version 2.2, the default sedimentation time for all Cell Types may be 90 seconds.
Sedimentation time can be adjusted via the Cell Type List window (see “
Creating a New Cell Type”).
4
S
elect the desired precision level in the Precision drop-down list. The precision level
determines the volume
to be analyzed. A higher analyzed volume will require a somewhat longer measurement time but more cells
will be counted, which will lead to a more precise calculation.
Minimum: approximately 3 images are used for the analysis.
Normal: approximately 6 images are used for the analysis.
Superior: approximately 8 images are used for the analysis.
Maximum: approximately 11 images are used for the analysis.
The precision names may appear differently if the system has been upgraded from some previous version
to Software version 2.2. In the case of a system that has been upgraded to version 2.2, the precision levels
may be called Low (corresponding to Minimum), Medium (corresponding to Normal), High (cor-
responding to Superior) and Very High (corresponding to Maximum). The same volumes are analyzed
for the corresponding levels.
One image in the Cedex HiRes Software 2.2 corresponds to approximately 25 images
in the Cedex HiRes
Software version 1.10. Thus a Minimum precision (3 images) roughly corresponds to 75 images of
Software version 1.10 of the Cedex HiRes Software, while Normal precision roughly corresponds to
150 images, Superior precision to 200 images and Maximum precision to 275 images.
5
Prepare the sample and attach the Cedex Sample Cup containing the sample to the sample port or place
it in the appropriate spot on the Multi Sample Tray (see “
Preparing the Sample and Attaching it to the Multi
Sample Tray or Sample Port
”, below).
■
Carrying out a Single Measurement
Defi ning the Measurement and Analysis (Process) Parameters
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