59
Operation
C
C
4.2.3. Performing a Prime
The Prime and HW Startup are performed as usual in the Liquid Management window as described in
“
Performing a HW Startup and Prime”. An empty Cedex Sample Cup must be placed in the DefaultCup position
(default position 1) of the Multi Sample Tray when running a Prime.
4.3 Carrying out Measurements with the Multi Sampler
Before carrying out measurements, ensure that the system is operational as described in “Checking the Analyzer”.
4.3.1. The Multi Run Data Entry Window
Click on the Multi Run button in the Shortcuts area of the Cedex Control Center or select Multi Run in the
Measurements menu. The Multi Run window will appear, and entries for sample data and parameters for the
analysis process are entered via this window (see Figure 45).
In addition to the entry columns for ReactorID, SampleID, Cell Type, Dilution and Precision, there are also
columns for Status and CupNr. The CupNr column shows the sample cup position on the Multi Sample Tray.
The Status column displays the state of the sample before, during and at the end of a measurement.
4.3.2. Preparing for Multiple Measurements in the Multi Run Window
The Multi Run window automatically displays all 20 possible sample cup positions. To set up and carry out
multiple measurements with the Multi Run do the following:
1
Transfer 300 µl of a cell suspension sample into Cedex Sample Cups and place the cups in the desired
locations on the Multi Sample Tray.
2
Click anywhere in the row of the MultiRun Measurements Preparation area that corresponds to the
location of a Cedex Sample Cup containing a sample. The row will be converted
to edit mode, and the sample
and process parameters for the measurement can be
defi ned (see “Opening the Measurement window and
specifying the Operating Mode and Sample Data
” and Figure 45).
3
Enter the desired sample data and parameters for the sample (see “Defi ning the Measurement and Analysis
(Process) Parameters
”).
4
Enter a comment in the fi eld Comment, if desired.
5
Repeat steps 2 – 4 for the remaining samples to be measured.
6
The rows in which entries are in the process of being fi lled out will remain in edit mode. To clear the entire
list of entries and start with a new list, click on the Clear List button on the right-hand side of the Multi
Run window.
■
Measurement of up to 20 samples in a row leads to prolonged incubation (up to 90 minutes) of cells at room
temperature. Tests in our lab using several different cell lines (CHO-K1 and several hybridoma cell lines)
showed no noteworthy infl uence of the prolonged incubation times on the measurement results. However,
this result may not necessarily apply to all cell lines available on the market. We, therefore, recommend test-
ing especially sensitive cell lines in advance to determine the number of samples that can be measured in a
row without affecting the cells.
Working with the Multi Sampler
Carrying out Measurements with the Multi Sampler
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