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Roche Cedex HiRes - Compactness Histogram

Roche Cedex HiRes
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Software
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4.6 Compactness Histogram
The ratio between the cell circumference and the cell area is based on an ideal sphere (4), which is equivalent
to the value >1< on the abscissa of the Cell Compactness Histogram. The extent of variation between the
cell shapes and an ideal sphere is refl ected in values being greater than 1. The shape of a cell is known as the
Compactness. Click on the Compactness Histogram button to display a diagram that compares viable (!) cell
shapes to that of an ideal sphere.
If a signifi cant number of cells (ordinate) have a high value, this could indicate improper handling of a sample.
The Compactness Histogram can be used to check the state of the cell culture, e.g. if the stirring speed is too
high or nutrient composition is incorrect.
Figure 61: Compactness Histogram
This diagram is based on the compactness of all viable cells in the measurement. To view results of individual
cell images, see Result Data area”.
Graphical Display of the Analysis Results
Compactness Histogram

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