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Cedex HiRes Analyzer Operator’s Guide
D
D
Administrator Functions
Adjustment of the Image Analysis using the Live Operator
7.5.3. Creating a New Cell Type
A new Cell Type must be created and saved via the Cell Type window before the Live Operator window can be
opened for modifi cation of that Cell Type.
A new Cell Type can be created by following the instructions below:
1
Open the Cell Type List window. Highlight the existing Cell Type upon which the new Cell Type should be
based.
2
Click on the New button. A message will appear asking if the new Cell Type should be based on the high-
lighted Cell Type in the list. Click on Ye s. The Cell Type window will open (see Figure 74). The Live Operator
button will be inactive at this time.
3
Fill in the information for:
The name of the new Cell Type
Sample preparation procedure (FVM is currently the only option)
Focus Offset: the offset from the ideal focus point.
The Focus Offset should be set to -25 to ensure best results for cell count and determination of
viability. Please consult the local Roche technical services before using any other number for the
Focus Offset as selecting another value for this parameter may lead to poor results.
Sedimentation Duration: the amount of time the cells are allowed to settle before chamber is scanned.
The default value for the Sedimentation Duration is 90 or 45 seconds, depending on the Cell
Type. This can be shortened depending on the Cell
Type. Please consult with local Roche technical
services before choosing other
sedimentation times. Roche support will provide instructions for
optimizing sedimentation times for specifi c cell lines.
4
Make sure that the IR Operator is set to CXHiResImageOperator.
The IR Operator DBDMOperator, if it is available, should not be selected and the parameters for this
operator should not be changed!
5
Click on the Save button. The Cell Type window will automatically close. The Cell Type List window will be
active again and include the just-created Cell Type in the list.
6
Highlight the row of the new Cell Type and click on the Edit button. The Cell Type window will reopen and
the Live Operator button will be active.
7
Click on the Live Operator button and the Live Operator window will open (see Figure 75).
8
In the Data Source area, select the Work Area, followed by the Reactor ID, Sample ID and Data Set
of the measurement for which the image analysis should be improved. The parameters for the loaded
measurement are preset.
9
Select an image of the measurement in the Image ID section. This image will be loaded and displayed in
the window in the upper right-hand corner with the living cells marked with a green circle and the dead cells
marked with a red circle.
10
Make the desired modifi cations on a single parameter fi rst. As soon as a parameter has been modifi ed,
reprocessing of the data is automatic (see “
Description of the Individual Operator Parameters”). The image
display then shows the reprocessed image (see “
Working with the Live Operator”).
11
When an optimized adjustment of the Operator parameters has been found, click on Save to confi rm the
modifi cation for the new Cell Type.
■
When Cancel is clicked on, the Live Operator window will close without saving the changes.
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