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Cedex HiRes Analyzer Operator’s Guide
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4.4 Object Diameter Histogram
Clicking on the Object Diameter Histogram button will bring up the Object Diameter Histogram (see Figure
60). Analogous to the representation of the diameter of the living cells, the diameter of all (!) recognized objects
is shown here, including objects such as cells, debris, air bubbles and viable and dead cell aggregates.
Figure 60: Object Diameter Histogram
4.5 Defi ning Diameter Intervals in the Diameter Histograms
The Cell and Object Diameter Histograms have three sliders that can defi ne separate diameter intervals. This
allows for dividing a diameter histogram into a maximum of four parts. The sliders can be activated by clicking
on the corresponding boxes and moving the sliders to the desired positions. One can also defi ne the margins of
the intervals by entering the numerical values on the left hand side of each slider.
The borders of the fi rst interval are defi ned by the minimum value and the fi rst numerical value entered. The
next two values defi ne the upper borders of the second and third intervals. The fourth interval is defi ned by the
upper border of the third interval and the maximum value.
The number of viable cells, their percentage of the total viable cell number and the viable cell concentrations [×
10
5
cells/ml] are listed in a table for each of the individual intervals. Similarly, the number of the objects analyzed,
their percentage compared to the number of all objects recognized, as well as the object concentration [× 10
5
objects/ml], are listed in a table under the Object Diameter Histogram for each of the individual intervals. The
intervals set in the Cell Diameter or Object Diameter histograms can be saved for future reference. In addition,
default intervals can be automatically set for all measurements via the User Preferences (see “Specifying Default
Measurement Parameters”).
Graphical Display of the Analysis Results
Defi ning Diameter Intervals in the Diameter Histograms
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