53
Operation
C
C
3.3 Preparing the Sample and Attaching it to the Multi Sample Tray or
Sample Port
Prepare the sample prior to measuring according to the following instructions:
1
Transfer the selected volume of a well-mixed cell suspension to a clean Cedex Sample Cup.
The Cedex HiRes Analyzer tolerates volume variations of +/- 10%. Any greater variation in sample
volume will lead to inaccurate results, and the Reagent Control monitor will no longer function
correctly. The Cedex HiRes Analyzer can
determine cell concentrations in the range of between
5× 10
4
– 1× 10
7
cells/ml.
Any higher concentration requires a manual dilution. Cells with a diameter
range of between 2 µm – 40 µm
can be assessed accurately. Object recognition performance lies
between 1 µm and 90 µm.
2
Remove the present Cedex Sample Cup from the Multi Sample Tray (or sample port). Attach the prepared
Cedex Sample Cup containing the cell suspension to the sample port (see Figure 40; the Cedex Sample Cup
is correctly attached to the sample port when it is in an upright position directly beneath it and the capillary
tube almost reaches the base of the Cedex Sample Cup) or place in the appropriate cup position on the
Multi Sample Tray (see Figure 42).
Figure 40: Attaching the Cedex Sample Cup to the sample port
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3.3.1. Starting the Measurement
Ensure that the entire measurement system, including the sample, is ready for a measurement
as follows:
1
Ensure that the Cedex Sample Cup is correctly placed in the Multi Sample Tray or attached to the sample
port.
2
Ensure that the reagent containers are suffi ciently fi lled and in their correct positions.
3
Ensure that the waste container is empty.
4
Click on Start Measurement in the middle of the Measurement window to begin the measurement.
■
After the sample has been injected into the chamber, the cells are allowed to settle to the bottom of the fl ow
chamber for a specifi ed amount of time (the sedimentation phase). The amount of time the cells are allowed to
sediment is set at 90 seconds by default for the Min. Size Cell Type and 45 seconds for Std. Size and Max. Size.
However, the sedimentation time can be changed to suit the needs of individual cell lines by creating a new Cell
Type (see “Adjustment of the Image Analysis using the Live Operator”).
The fl ow chamber is checked for impurities with a separate scan ( Prescan) during the sedimentation phase
(see “Viewing the Prescan Image”). If it is ascertained that there are impurities in the fl ow chamber, an error
message, “Dirt or bubbles detected during prescan!” will appear. The measurement will be completed and the
results calculated even in the case of a Prescan failure. However, when the measurement results are viewed in
the Measurement window there will be no check in the Valid checkbox below the Image area. (see Figure 41).
Carrying out a Single Measurement
Preparing the Sample and Attaching it to the Multi Sample Tray or Sample Port
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