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Operation
C
C
3.1 Opening the Measurement window and specifying the Operating Mode
and Sample Data
Open the Measurement window either by clicking on the Measure button in the Shortcuts area or by selecting
Single Measurement in the Measurements menu. The entry areas for choosing the desired operation and
specifying the sample data are located in the Sample Parameters and Processing parameters areas of the
Measurement window. The parameters should be entered just before measuring in order to ensure a direct
relationship between the sample and the data.
Follow the instructions below to specify sample data for a single measurement:
1
Open the Measurement window by clicking on the Measure button in the Shortcuts area or by selecting
Single Measurement in the Measurements menu.
2
Enter a clearly defi ned Reactor ID to describe the sample (e.g., the name of the cell culture) in the Reactor
ID area. You may either choose a Reactor ID from the drop down list or enter a new Reactor ID. A new entry
can be defi ned by up to 20 alphanumerical symbols.
3
Enter a clearly defi ned Sample ID in the Sample ID area (e.g., P001). The Sample ID can be freely defi ned.
If the intended measurement is not the fi rst in a row of measurements, i.e. if it has the same Reactor ID
as a previous measurement, then the software will automatically enter the consecutive Sample ID into the
Sample ID area (e.g. P001, P002,...). In that case, it is not necessary to enter the Sample ID manually unless
a different Sample ID is desired.
4
If desired, use the Sample Drawing drop-down menu to select the date when the sample was drawn. Note
that the process date and time, i.e. the date and time the sample was measured, is automatically entered.
5
Choose the appropriate dilution factor from the Dilution drop-down list (e.g., 1:3, 1:2, 1:5… etc.).
When a dilution of 1:2 is prepared, then 1 part cell suspension has been added to 1 part dilution medium.
A dilution of 1:5 means that 1 part cell suspension has been added to 4 parts dilution medium, etc. For
undiluted samples, a dilution factor of 1:1 should be entered. It may be necessary to dilute samples with
very high cell densities ( >1 × 10
7
cells/ml) manually prior to measuring. The dilution ratio chosen from
the drop down list only relates to the manual dilution of the sample prior to placing the sample on the
system. Dilutions that are an automatic part of the measurement process in the analyzer are already
taken into account by the Cedex HiRes Software. The calculations are carried out automatically and have
nothing to do with the entered dilutions.
6
Select the sample volume of 300 µl in the Volume drop-down menu. Currently, only the 300 µl option is
available for the Cedex HiRes Analyzer.
7
The System name, as well as the process date and time of the assignment, will be automatically transferred
to the “Processing parameters” area in the Measurement window.
8
Entering a comment for a measurement is optional. Enter the remarks in the Comment box on the Sample
Parameters area, if desired.
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Carrying out a Single Measurement
Opening the Measurement window and specifying the Operating Mode and Sample Data
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