49
Operation
C
C
To refi ll individual containers with reagents, follow the instructions below:
1
Rinse all reagent containers well before refi lling! Ensure there are no impurities such as dust or fl uff in the
containers.
2
Fill the detergent container to the required level with Cedex Detergent (see label).
Only Cedex Detergent or a detergent with identical specifi cations should be used for the cleaning
procedures in order to prevent damage to the sensitive surface of the Liquid Management components.
3
Fill Roche Diagnostics GmbH Cleaning Solution in the Cleaning Solution container to the required level
(see label).
4
Fill distilled or de-ionized water in the water container to the required level (see label).
5
Fill 0.2% Trypan Blue solution in the Trypan Blue container to the required level (see label). Roche Diagnostics
GmbH recommends using 0.2%Trypan Blue solution. If necessary, dilute a more concentrated Trypan Blue
solution with buffer (Phosphate Buffered Saline (PBS), pH 7.4).
6
Place all of the reagent containers, as well as the emptied waste container, in the appropriate positions in the
Reagent Tray (see Figure 38).
7
A Cedex Sample Cup should always be attached to the single sample port (if a single sample port is
available; see Figure 40). The Cedex Sample Cup (4 ml) is correctly attached when it is in an upright position
beneath the sample port and the capillary tube almost reaches the bottom of the Cedex Sample Cup. In the
case of a system with a Multi Sampler, the Cedex Sample Cup must be placed in the DefaultCup position on
the Multi Sample Tray (see “
The Multi Sampler”).
If the Trypan Blue is completely used up during measurements and then refi lled, a Prime must be
performed again before running the next sample (see “
Performing a HW Startup and Prime”). If a
Prime is not performed, the Prescan of the fi rst measurement after refi lling of Trypan Blue will fail and
the measurement will be considered invalid (there will be no check in the Valid checkbox found in the
Measurement window (see ”
Viewing the Prescan Image”). Subsequent measurements will be valid.
■
2.6 Ch ecking the Analyzer
Since measurement results obtained with the Cedex HiRes Analyzer depend on correctly fi lled reagent containers
and the proper performance of the analyzer, the user should check the analyzer for possible wear and tear, and
also check whether the reagent containers have the correct volume prior to each set of measurements, as follows:
1
Check the syringe module (2.5 ml syringe and 8-way valve) for wear and tear or contamination. Renew the
syringe if required (see “
Syringe Maintenance”).
2
Check the number of runs possible. This is listed in the right-hand corner of the Control Center next to a
green or red circle. Change the Reagent Kit punctually. Observe the reminder to change the Reagent Kit
(see “
Installing or Changing the Reagent Kit“).
3
Check whether there is an empty Cedex Sample Cup attached to the sample port or a Cedex Sample Cup
in the DefaultCup position on the sample tray in the case of a Multi Sampler. If there isn’t, attach one
(see Figure 40 or “
The Multi Sampler“).
4
Perform a Hardware Management (HW) Startup if necessary.
5
Perform a Prime if a Reagent Kit is installed and new measurements are to be carried out (see “Performing a
HW Startup and Prime
”).
6
Have the cell suspension samples or particle solutions ready for the analysis.
■
Preparing the Analyzer for Measurements
Checking the Analyzer
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