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Roche Cedex HiRes - Cultivation Time Chart

Roche Cedex HiRes
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Software
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4.2 Aggregate Histogram
The Cedex HiRes Analyzer offers an evaluation of aggregates in cell cultures. Clusters of up to 15 cells are
displayed. Click on the Aggregate Histogram button in the Histograms window to open the corresponding
histogram window.
The Aggregate Histogram shows the number of cells in the cell culture found in aggregates, as well as how
many cells each aggregate is composed of. Both the actual cell number and the percentage distribution can be
displayed.
Figure 55: The Aggregate Histogram, detailed view (divided into living and dead cells), here: (absolute) number of cells
The Number of Cells [#] identifi ed is displayed on the y-axis and at the top of the bars. The Aggregate Size, in
terms of the number of cells per aggregate, is given on the x-axis. Figure 55 shows, for example, that 148 living
and 40 dead cells of all identifi ed cells were found in aggregates of two cells.
Graphical Display of the Analysis Results
Aggregate Histogram

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