Roche Cedex HiRes - Performing a HW Startup and Prime

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Cedex HiRes Analyzer Operator’s Guide
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2.4 Performing a HW Startup and Prime
Two elements of the analyzer are usually shut down at the end of the day: the Hardware Management and
the Liquid Management. When the Hardware Management is shut down, several elements of the analyzer,
such as the scanner, Multi Sampler and pump, are not yet initialized. A HW Startup will initialize hardware
interfaces and must be done before a measurement can be performed. If a measurement is attempted before the
Hardware Management has been started, an error message will appear, “Cannot start a new process: Hardware
Management is not started”. The HW Startup may be automatically carried out when the software is launched
if the Administrator has set this up in the system options.
In the case of the Liquid Management, the capillary tubes and syringe are fi lled with air or water when the
analyzer is fi rst installed or any time an LM Shutdown has been performed (see, “Carrying out an LM Shutdown
and HW Shutdown”). Performing a Prime will fi ll the capillary tubes and 8-way valve with the appropriate
reagents. All measurements are based on the capillary tubes having a precise amount of reagents in them.
Not performing a Prime will lead to cell samples being incorrectly stained and, consequently, inaccurate
measurement results.
A Prime must, therefore, be performed after an LM Shutdown has been executed in order to run a measurement.
Since LM Shutdown should be run at the end of the previous day, a Prime is necessary prior to the fi rst
measurement of the day. No measurement is possible without performing a Prime fi rst. If a measurement is
attempted before the machine has been primed, an error message will appear, “Cannot start new process: system
is not primed”.
Both the HW Startup and the Prime functions are located in the same window. Follow the instructions below
to perform a HW Startup and Prime:
1
Click on the HWM button in the Cedex HiRes Control Center or else select Hardware Management
under the Functions menu. The Hardware Management window will open.
2
If the Hardware Management has not been started, the current status fi eld will have the message “HW not
started and LM not primed”.
3
Click on HW Startup if required. The Hardware Management will start and the current status fi eld will have
the message “HW started and LM not primed”.
4
Ensure that a Reagent Kit is installed (see “Preparing the Reagent Kit”) and a clean, empty Cedex Sample
Cup is correctly positioned on the Multi Sample Tray in the DefaultCup position (see “
The Multi Sampler
DefaultCup
”) or attached to the single sample port.
5
Click on Prime. The duration of a Prime procedure is displayed in the Estimated total time area. The time
displayed is an estimate based on the time required for the previous Prime. The message “HW started and
LM primed”, appears in the current status fi eld when the Prime has been completed.
6
Click on to close the window.
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There should not be a break of more than 2 hours between a Prime and a measurement. If more than 2 hours
have passed since the last measurement, a new Prime must be performed.
Preparing the Analyzer for Measurements
Performing a HW Startup and Prime