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Maintenance and Care
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Standard Clean (with cleaning solution, detergent, and distilled water for approx. 90 seconds)
The entire Liquid Management system is rinsed with distilled water, cleaning solution, detergent and fi nally
with distilled water again. This routine removes dirt particles and is used after measuring a set of samples or
before a longer pause.
Intensive Clean (with cleaning solution, detergent, and distilled water for approx. 20 minutes)
The fl ow chamber is thoroughly rinsed with distilled water + cleaning solution + distilled water + detergent
and fi nally distilled water. The detergent remains in the fl ow chamber for a longer period to work on resistant
particles.
The Intensive Clean routine is used on weekly basis to remove heavy and resistant contamination of the fl ow
chamber, capillaries tubes, syringe and 8-way valve. Even the smallest dirt particle attached to the glass wall
of the fl ow chamber can negatively infl uence a measurement. See also “
Scanning the Chamber to Verify the
Effectiveness of Cleaning Routines”. If measurement values within a sample measurement show large variations,
this could indicate that the fl ow chamber is soiled.
Intensive Clean with HCl
A special Intensive Clean is required twice per month for a thorough cleaning. For this cleaning, a solution of
0.1 – 1 N HCl is used instead of detergent.
Follow the instructions below:
1
Remove the Detergent container and replace it with an appropriate container with a 0.1 – 1 N HCl solution,
taking care that the capillary tube reaches far enough into the solution.
2
Start Prime.
3
Start Intensive Clean after Prime has ended.
4
Replace the HCl container with the Detergent container at the end of the Intensive Clean process.
5
Start Prime again.
6
Start Standard Clean after Prime has ended.
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The Chamber Clean 1 and Chamber Clean 2 cleaning routines are only required for the fl ow chamber.
Chamber Clean 1 (with water for approx. 10 seconds)
The fl ow chamber is rinsed at high pressure with distilled or de-ionized water being drawn in through the
syringe. Use this routine to remove resistant protein aggregates, fl uff particles or other obstructions from the
fl ow chamber.
Chamber Clean 2 (with water, detergent, and air for approx. 30 seconds)
This routine is more intense than the Chamber Clean 1 procedure. Once the fl ow chamber is rinsed with water
and detergent, the remaining liquid in the capillary tubes between the syringe and valve head is drawn through
the fl ow chamber and then pressed back again with compressed air.
Use this routine twice daily to remove resistant protein aggregates, fl uff particles or other obstructions from the
fl ow chamber.
Daily and Manual Maintenance and Cleaning Routines
Cleaning Routines Carried out by the Liquid Management
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