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Maintenance and Care
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The automatic adjustment and verifi cation of alignment is automatically carried out in several steps. First,
water is pumped into the fl ow chamber and a white balance is carried out. The Cedex Calibration Beads are
then injected into the fl ow chamber. Focus fi nding occurs after sedimentation. An automatic cleaning of the
fl ow chamber and the capillaries, as well as a new white balance, is done after focus adjustment. The focus
adjustment’s time-progression is displayed as an estimated value in the form of a blue progression bar.
Scans are carried out for every focus position. The images of the four corner areas are analyzed and these focus
values displayed in a diagram (see Figure 80). Focus positions are shown on the x-axis and focus quality on the
y-axis. A measurement can only deliver optimal results if the fl ow chamber is scanned at one level. This means
that the corner areas must have the same focus level.
The result appears on the monitor as a message box once the focus adjustment is fi nished (see Figure 81). Verify
that the different positions of the fl ow chamber have the same focus position by checking against the conditions
explained below, and confi rm your approval with Ye s .
The focus position automatically set for the four corner areas (optimal position) must lie between 850 and
1250. Otherwise, an authorized service technician must re-adjust the Cedex HiRes Analyzer. Another important
value is the allowed maximum difference between the optimal focus values of the four corner areas. The focus
difference must be between 0 and 20. Otherwise, an authorized service technician must re-adjust the Cedex
HiRes Analyzer.
The Automatic Adjustment and Alignment Report can be printed out via the Print Report button. The report
shows a diagram (Focus Chart) with four curve progressions to the focus position.
The quality of the automatic adjustment and verifi cation of alignment depends on the pre-adjustment of the
fl ow chamber and an even concentration of the focus beads in all of the pictures of the 4 corners. In order to
check that these conditions have been fulfi lled, the following must be verifi ed:
No edges are visible in the 4 pictures of the corners of the fl ow chamber.
The pictures are not out of focus.
All of the pictures have an even distribution of beads with at least 50 beads in every picture.
The focus curves of all 4 corners have two clear maximums with one clear, marked minimum in the middle.
If any one of these conditions is not fulfi lled, the results of the automatic focus adjustment should not be used!
Should stripy shadows or lines appear on the images when running measurements, a new focus adjustment
needs to be run as poor image quality can affect the results of the measurement.
Automatic Adjustment and Alignment Verifi cation
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