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ANALYSIS
INTRODUCTION
pocH-100
i
Revised February 2011
2.1.8. Histogram flags
The pocH-10 0
i
extracts the characteristics of the histogram and displays them as histogram flags.
If there are histogram flags, repeat analysis. If flags are still displayed, one of the following problems may apply.
Flag Probable sample cause
Correction
Check
smear.
Warm sample
and repeat
analysis.
Manual
count.
Wash
blood
cells.
WL
Incomplete lysing of red blood cells, presence of nucleated red blood cells, in-
crease in large platelets, platelet aggregation or agglutination, precipitation of
fibrin, presence of proteins or lipids.
If incomplete lysing is suspected, perform a 1:5 dilution of the
sample (50 μL of whole blood added to 200 μL of diluent) and
re-analyze. Adjust the results for the dilution factor.
RL
Presence of fragmented red blood cells, increase in large platelets, platelet aggre-
gation or agglutination, presence of micro-erythrocytes.
PL
Effects of cryoglobulins, fragmented red blood cells, or cellular fragments of white
blood cells.
WU
Incomplete lysing of red blood cells, presence of immature white blood cells,
white blood cell aggregation, platelet satellite phenomenon, etc.
If incomplete lysing is suspected, perform a 1:5 dilution of the
sample (50 μL of whole blood added to 200 μL of diluent) and
re-analyze. Adjust the results for the dilution factor.
RU
Effects of cold agglutinin, inclusion of white blood cells.
PU
Increase of large platelets, inclusion of fragmented red blood cells, precipitation
of cryoglobulins, platelet aggregation or agglutination, presence of micro-erythro-
cytes.
DW (RBC)
Significant anisocytosis, etc.
DW (PLT)
Inclusion of fragmented red blood cells, nonuniformity in size of platelets, effects
of cryoglobulins, etc.
If cyroglobulins are suspected, first warm the sample and
repeat analysis. If error message persists, perform a plasma
replacement (remove plasma and replace with equal volume
of diluent) and repeat analysis.
MP (RBC)
Effects of anemia treatment or blood transfusion causing the presence of cells of
multiple sizes.
MP (PLT)
Platelet aggregation, low platelet count.
T1
Incomplete lysing of red blood cells, etc., causing the first two WBC populations
in the WBC-Histogram not to be separated, presence of CML or other immature
granulocytes.
If incomplete lysing is suspected, perform a 1:5 dilution of the
sample (50 μL of whole blood added to 200 μL of diluent) and
re-analyze. Adjust the results for the dilution factor.
T2
Aged sample, incomplete lysing of red blood cells, etc., causing the last two WBC
populations in the WBC-Histogram not to be separated, presence of CML or other
immature granulocytes.
F1, F2, F3
Sample with high values for monocytes, eosinophils, and basophils, incomplete
lysing of red blood cells, aged sample, etc., presence of CML or other immature
granulocytes.
AG
Presence of nucleated red blood cells, increase of large platelets, platelet aggrega-
tion or agglutination, precipitation of fibrin, presence of proteins or lipids, etc.
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