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pocH-100
i
Revised June 2008
SETTINGS & CALIBRATION
INTRODUCTION
5.2. CALIBRATION
5.2.1. Calibrate the instrument
• If quality control shows repeated deviations in the same direction.
• If a major component of the instrument has been replaced.
HGB Determination of hemoglobin concentration (DIN 58931)
HCT Determination of the concentration of blood corpuscles in blood (DIN 58933)
Recommended measuring methods:
Establishing reference values
The HGB and/ or HCT values are corrected by a calibration value.
• Calibration does not need to be performed at
specific intervals. Follow your internal laboratory
regulations for performing calibration.
• Each sample should be analyzed at least three
times.
• EIGHTCHECK is not suitable for calibration pur-
poses, but for quality control instead.
Important
Samples used for calibration
Use 5 or more samples of fresh blood meeting the following conditions:
• Blood from healthy person who is not taking any medication
• Blood added with an appropriate amount of anticoagulant
• Per-sample whole blood volume to exceed 2 mL
• HGB value to exceed 10.0 g/dL
• HCT value to be within 35.5% and 55.5%
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