Thermo Scientific QuantStudio 1 - CHAPTER 4 Create and Run Experiments on the Instrument; Run an Experiment; Create and Run an Experiment from a Template; Run an Experiment from a Saved File

Run an experiment
1.
In the home screen, touch Open Template.
2.
(Optional) Touch a category in the left column.
3.
Touch the le name.
4.
(Optional) Enter or edit template properties, including Run File Name (EDS le
name), Plate Barcode, Reagent Information, and Data Destination (see “Enter
or edit template properties“).
5.
(Optional) Edit the run method (see “Edit the run method“ on page 43).
• Add, remove, or edit a step, stage, melt curve, or data collection point.
• Adjust the heated cover temperature, sample volume, or number of cycles.
• Congure ramp rate and pause seings.
6.
(Optional) Dene plate wells with sample names and view Well ID, Targets, or
Dyes (see “Dene, assign, and view well details“).
7.
Load a plate into the instrument (see “Load and unload a plate in the
QuantStudio
™
1 Real-Time PCR Instrument“ on page 22).
8.
Touch Start Run.
When prompted, conrm that you inserted a plate.
Note: To disable this reminder, select Do not show again or select
Seings4Insert Plate Reminder in the home screen.
1.
In the home screen, touch Load Experiment.
2.
Touch the appropriate icon to navigate to your le location.
• For les saved to the guest prole, touch My Instrument4Public.
• For pre and post read les, touch My Instrument4Post Read.
3.
Touch the le name.
4.
(Optional) Manage templates in either USB or My Instrument (see
“Manage templates (EDT les)“).
5.
(Optional) Enter or edit template properties, including Run File Name (EDS le
name), Plate Barcode, Reagent Information, and Data Destination (see “Enter
or edit template properties“).
6.
(Optional) Edit the run method (see “Edit the run method“ on page 43).
• Add, remove, or edit a step, stage, melt curve, or data collection point.
• Adjust the heated cover temperature, sample volume, or number of cycles.
• Congure ramp rate and pause seings.
Create and run an
experiment from a
template
Run an
experiment from a
saved file
Chapter 4 Create and run experiments on the instrument
Run an experiment
4
40
QuantStudio
™
1 Real-Time PCR System Installation, Use, and Maintenance Guide