Thermo Scientific QuantStudio 1 - Calibrate the Custom Dye

Create a custom dye calibration plate
IMPORTANT! Wear powder-free gloves while creating the dye plate.
Create a full plate of the custom dye diluted to the optimal concentration:
1.
Dilute the custom dye to the optimal concentration in buer.
Prepare an adequate volume, using a volume range of 10–20 µL/well for a
96-well plate.
2.
Pipet the appropriate volume of the diluted custom dye to the plate wells.
3.
Seal the plate.
4.
Vortex the plate for 5 seconds, then centrifuge at 750–1,000 × g for 2 minutes.
5.
Conrm that the liquid in each well is at the boom of the well and free of
bubbles. If it is not, centrifuge the plate again.
IMPORTANT! Keep the boom of the plate clean. Fluids and other contaminants
on the boom of the plate can contaminate the sample block and cause an
abnormally high background signal.
Add a custom dye to the instrument
1.
In the instrument home screen, touch
Seings4Maintenance and
Service4Calibrations4Custom4Custom Dye.
2.
Touch Add Custom Dye.
Calibrate the
custom dye
Chapter 5 Calibrate and verify instrument performance
Calibrate custom dyes
5
QuantStudio
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1 Real-Time PCR System Installation, Use, and Maintenance Guide
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