Thermo Scientific QuantStudio 1 - Perform Instrument Verification Using Rnase P Plates; Instrument Verification Description

IMPORTANT! Wear powder-free gloves while creating the background plate.
1.
Remove a reaction plate from its box and place it on a clean, dry surface.
2.
Aliquot 50 µL of deionized water to each well of the reaction plate.
3.
Seal the plate using an optical adhesive cover or optical at caps.
4.
Use the plate for background calibration.
Perform instrument verification using RNase P plates
Instruments are factory-calibrated, so calibration is not necessary for initial
installation. However, perform instrument verication:
• After installation and before rst use of the instrument.
• After performing instrument calibrations.
• As needed to conrm instrument performance.
The instrument requires valid ROI/uniformity, background, and dye calibrations to
perform instrument verication.
Instrument verification description
Purpose
Description Pass criteria
Confirms the
performance of the
instrument.
Quantifies the number of copies of
the human RNase P gene in samples
with known concentrations of the
corresponding DNA template.
The instrument passes performance specifications
if the following inequality is true and the
instrument successfully distinguishes between
unknown populations A and B with a statistical
confidence level of 99.7%.
[(C
tA
) – 3(σ
CtA
)] > [(C
tB
) + 3(σ
CtB
)]
where:
• C
tA
= Average C
t
of unknown population A
• σ
CtA
= Standard deviation of unknown
population A
• C
tB
= Average C
t
of unknown population B
• σ
CtB
= Standard deviation of unknown
population B
Chapter 5 Calibrate and verify instrument performance
Perform instrument verification using RNase P plates
5
QuantStudio
™
1 Real-Time PCR System Installation, Use, and Maintenance Guide
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