Calibration Recommended frequency
Background
• Every two years (recommended)
• Background calibration can also be performed, as needed:
– To check for contamination (depends on usage and
laboratory conditions).
– To obtain the most accurate data for the removal of
background fluorescence.
Note: Performing a Background calibration does
not
invalidate
any other calibration.
Dye
• Every two years (recommended)
• During a Dye calibration, only the dyes on the given spectral
calibration plate are calibrated.
Note: Performing a Dye calibration for a given dye plate does
not
invalidate any other calibration.
RNase P instrument
verification
• After installing or moving the instrument
• After performing instrument calibrations
• As needed to confirm instrument performance
Note: To prepare custom dye plates and to perform custom calibrations, see
“Calibrate custom dyes“ on page 62.
Calibration descriptions
Calibration description and purpose
Pass Criteria
ROI/Uniformity
• The software captures images for each optical filter.
• The software uses calibration data to map the increase in fluorescence
to the plate wells during subsequent runs and to evaluate well-to-well
consistency of the signals.
The image for each filter
distinguishes all wells of the plate.
Each well in the image is distinct.
Background
• The software captures background images for each optical filter in the
absence of sample and reagent, and it checks that the fluorescence from
each well is below a fluorescence threshold.
• The software uses calibration data to remove background fluorescence
during a run.
Note: You can also run this calibration to determine if contamination is
related to the sample block or the plate.
The plate images for all filters are
free of abnormal fluorescence.
Chapter 5 Calibrate and verify instrument performance
Calibration descriptions
5
50
QuantStudio
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1 Real-Time PCR System Installation, Use, and Maintenance Guide
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