7.
(Optional) Dene plate wells with sample names and view Well ID, Targets, or
Dyes (see “Dene, assign, and view well details“).
8.
Load a plate into the instrument (see “Load and unload a plate in the
QuantStudio
™
1 Real-Time PCR Instrument“ on page 22).
9.
Touch Start Run.
When prompted, conrm that you inserted a plate.
Note: To disable this reminder, select Do not show again or select
Seings4Insert Plate Reminder in the home screen.
This feature applies only to runs started from the instrument and is not available for
runs started from the desktop software. If you are signed-in, this feature applies to the
last run from your instrument prole.
1.
In the home screen, touch Run Last.
2.
(Optional) Enter or edit template properties, including Run File Name (EDS le
name), Plate Barcode, Reagent Information, and Data Destination (see “Enter
or edit template properties“).
3.
(Optional) Edit the run method (see “Edit the run method“ on page 43).
• Add, remove, or edit a step, stage, melt curve, or data collection point.
• Adjust the heated cover temperature, sample volume, or number of cycles.
• Congure ramp rate and pause seings.
4.
(Optional) Dene plate wells with sample names and view Well ID, Targets, or
Dyes (see “Dene, assign, and view well details“).
5.
Load a plate into the instrument (see “Load and unload a plate in the
QuantStudio
™
1 Real-Time PCR Instrument“ on page 22).
6.
Touch Start Run.
When prompted, conrm that you inserted a plate.
Note: To disable this reminder, select Do not show again or select
Seings4Insert Plate Reminder in the home screen.
Edit an experiment before starting a run
Access a template (EDT le). For more information, see the following sections.
• “Create and run an experiment from a template“ on page 40.
• “Run an experiment from a saved le“ on page 40.
• “Repeat the last instrument run“ on page 41.
In the Properties screen, touch Edit to enter or edit template properties.
• Edit the le name for the EDS le for the run.
a.
Touch the Run File Name eld.
Repeat the last
instrument run
Enter or edit
template
properties
Chapter 4 Create and run experiments on the instrument
Edit an experiment before starting a run
4
QuantStudio
™
1 Real-Time PCR System Installation, Use, and Maintenance Guide
41