Thermo Scientific QuantStudio 1 - Perform Calibrations

Thaw, vortex, and centrifuge a calibration plate
1.
Remove the calibration plate from the freezer, then thaw the plate in its
packaging. Keep plates protected from light until you perform the calibration.
• Thaw each plate for 30 minutes.
• Use each plate within 2 hours of thawing.
IMPORTANT! Do not remove the plate from its packaging until you are ready to
use it. The uorescent dyes in the wells of calibration plates are photosensitive.
Prolonged exposure to light can diminish the uorescence of the dyes.
2.
While wearing powder-free gloves, remove the calibration plate from its
packaging and retain the packaging. Do not remove the optical lm.
3.
Vortex the plate for 5 seconds, then centrifuge at 750–1,000 × g for 2 minutes.
4.
Conrm that the liquid in each well is at the boom of the well and free of
bubbles. If it is not, centrifuge the plate again.
IMPORTANT! Keep the boom of the plate clean. Fluids and other contaminants
on the boom of the plate can contaminate the sample block and cause an
abnormally high background signal.
1.
In the home screen, select a calibration to perform.
Calibration Touch
ROI/Uniformity
[1]
Settings4Maintenance and Service4Calibrations4ROI and
Uniformity
Background
[2]
Settings4Maintenance and
Service4Calibrations4Custom4Background
Dye Settings4Maintenance and Service4Calibrations4Dye
[1]
Automatically followed by Background calibration.
[2]
Initiate via this route if performing Background calibration only.
2.
Follow the instructions on the screen to start the calibration.
Note: Dye calibration only: Select the Dye Plate to run, then touch Next.
3.
Load the plate into the instrument.
4.
Touch Start.
Perform
calibrations
Chapter 5 Calibrate and verify instrument performance
Perform ROI/uniformity, background, and dye calibrations
5
QuantStudio
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1 Real-Time PCR System Installation, Use, and Maintenance Guide
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