Thermo Scientific QuantStudio 1 - Troubleshoot Verification Failure

Observation Possible cause Recommended action
Verification failed The plate was improperly
prepared.
Ensure the following:
• The correct plate was used for the
verification performed.
• The plate was properly thawed.
• The plate was properly centrifuged.
• The plate was properly sealed.
Open the EDS file for the verification in the
desktop software or cloud software to view the
flags and troubleshooting details for failed
wells.
The plate is damaged or
contaminated.
Check for damage, improper plate seal, or
contamination.
Order a replacement plate. If the replacement
plate fails, contact Support.
High fluorescence signal The reaction volume is not
correct.
Ensure that reaction volumes in the plate are
correct and match the volume that is entered
in the Method tab.
Signals that exceed the limit of
normal fluorescence may
indicate fluorescent
contaminants on the plate or
the sample block.
Examine the bottom of the reaction plate. If
there is contamination, prepare a new plate
and run the experiment again.
Identify the location of contamination on the
plate or sample block.
1. Obtain or prepare a background plate (see
page 56).
2. Follow the procedures that are described
in “Identify contamination“ on page 56.
Verification failed but plate is
undamaged
The incorrect plate was used
for verification.
Use the correct RNase P plate for verification.
The plate was improperly
prepared.
Repeat the verification with a new properly
prepared plate.
Note: The verification procedure is an
experiment run, so each RNase P plate can
only be used once.
Open the EDS file for the verification in the
desktop software or cloud software to view the
flags and troubleshooting details for failed
wells.
If the verification fails again, order a
replacement plate. If the replacement plate
fails, contact Support.
Troubleshoot
verification failure
Chapter 5 Calibrate and verify instrument performance
Perform instrument verification using RNase P plates
5
QuantStudio
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1 Real-Time PCR System Installation, Use, and Maintenance Guide
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