Thermo Scientific QuantStudio 1 - Calibrate for a Custom Melt Curve Experiment

Calibrate for a custom melt curve experiment
Note: A custom melt calibration calibrates a custom dye and a melt calibration at the
same time.
Before running the custom melt calibration complete the following tasks:
• Ensure that all calibrations are current (touch Seings4Maintenance and
Service4Calibrations4History and Reminders).
• Add a custom dye to the instrument (see “Add a custom dye to the
instrument“ on page 65).
1.
In the home screen, touch Seings4Maintenance and
Service4Calibrations4Custom4Custom Melt.
2.
Touch PCR + Melt or Melt only as appropriate for the kit you are using.
3.
Load the plate into the instrument.
4.
Select or add a dye, then select a lter set appropriate for your dye's wavelength
(see lter-wavelength table below).
Note: Refer to your reagent kit documentation for dye name and wavelength
information.
Peak filter
Filter wavelength (nm)
[1]
Excitation Emission
x1-m1 470 ± 15 520 ± 15
x2-m2 520 ± 10 558 ± 12
x4-m4 580 ± 10 623 ± 14
[1]
The central wavelengths are the optimized wavelengths.
IMPORTANT! If the selected lter set does not match your reagent kit
documentation, then the incorrect wavelength may be collected during a run.
5.
(Optional) Touch Reagents, then enter reagent information.
6.
Touch Start.
7.
When the run is complete and the screen displays Calibration Complete, touch
View Results4Details.
Chapter 5 Calibrate and verify instrument performance
Calibrate for a custom melt curve experiment
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QuantStudio
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1 Real-Time PCR System Installation, Use, and Maintenance Guide