Thermo Scientific QuantStudio 1 - Perform Roi;Uniformity, Background, and Dye Calibrations; Workflow: Calibration; Prepare a Calibration Plate

4.
In the Calibrations History tab:
• View the history of each calibration type.
• (Optional) Click to download the calibration history report.
Perform ROI/uniformity, background, and dye calibrations
Perform an ROI/uniformity calibration
You are automatically prompted to perform background calibration.
▼
Perform a background calibration
Perform any time that ROI/uniformity calibrations are current.
▼
Perform system dye calibrations
Perform any time that ROI/uniformity and background calibrations are current.
▼
( Optional ) Perform custom dye calibrations (“Calibrate custom dyes“ on page 62)
Perform any time that ROI/uniformity and background calibrations are current.
Materials required for calibration plate preparation
• Plate(s) for the calibration that you are performing:
– ROI/Uniformity plate (one ROI plate needed)
– Background calibration plate
– Dye calibration plates
Note: We recommend calibrating with all Spectral Dye Calibrations Plates
available for your block conguration even if you are not using all the dyes
in the plates.
Note: Do not discard the packaging for the calibration plates. Each calibration
plate can be used up to 3 times if the following conditions are met:
·
The plate is stored in its packing sleeve at –15 to –25°C.
·
The plate is used within 6 months after opening.
·
The plate is used before the plate expiry date
• Centrifuge with plate adapter; buckets cleaned before use
• Powder-free gloves
• Safety glasses
Workflow:
Calibration
Prepare a
calibration plate
Chapter 5 Calibrate and verify instrument performance
Perform ROI/uniformity, background, and dye calibrations
5
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QuantStudio
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1 Real-Time PCR System Installation, Use, and Maintenance Guide